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For The only histidine substitutions, the N61H mutant is similar in catalytic action on the double mutant whereas the Q59H mutant is diminished by below an buy of magnitude relative to the wild form enzyme. To the substitutions at Asn-61, the substitution having an alanine is significantly fewer disruptive compared to change to the histidine.
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m with the E131Q mutant is enhanced to 3.six mM. A significant portion of the reduction in catalytic efficiency has arisen since the metallic written content in the purified proteins is fairly low.
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m although the Zn enzyme has the lowest. Comparable developments while in the choice of the catalytic constants ended up claimed previously for the Co, Zn, Mn and Fe reconstitutions of apo-NagA from E. coli
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